We reinvented micro HPLC with new technological advantages that provide superior results and increased productivity.
Microfluidic Flow Control (MFC)
MFC works by continuously measuring the flow rate of each mobile phase. An embedded processor uses the flow rate measurements to control each pneumatically driven pump. Full gradients can be run as rapidly as 10 seconds with excellent reproducibility. MFC improves separation speed and flow stability.
Optimized MS Interface-Low Dispersion Electrodes & Column Mounting Hardware
Specially designed electrospray ionization electrodes for use with the AB SCIEX TurboV™ Source minimize peak broadening and maximize resolution and sensitivity. A hardware kit for use with the ekspert™ microLC 200 System and ExpressHT™-Ultra System enables closer mounting of heated columns to the Turbo V source to minimize tubing lengths and further maximize chromatographic resolution. When the new electrodes are combined with the new column heater mounting hardware, peak dispersion is minimized from the LC all the way into the mass spectrometer.
The advantages of microLC over conventional HPLC disappear unless there's a proportional reduction in fluid mixing volume. Eksigent's microscale mixer has a volume of just 300 nL, and a mixing delay of only 2 seconds at 10 uL/min. This rapid mixing virtually eliminates the gradient delays inherent in conventional systems at the start of the method and at re-equilibrations. The rapid "deterministic" mixing leads to extremely precise gradients, rapid system response, and high gradient linearity.
Variable Injection System
Our variable injection system utilizes an electronically controlled injection valve coupled with the MFC flow delivery system to deliver precise nanoliter injection volumes. A standard autosampler fills a sample loop (typically 250 nL) with sample. The valve is then toggled from load to inject and back to the load position, based on integrating the flow rate over time to reach the desired injection volume. This solution provides high precision, software-selectable injection volume control, and compatibility with the popular CTC Analytics autosampler system.
Microscale UV Flow Cell Detection
Simply scaling down a conventional UV absorbance detection system to capillary levels results in increased dispersion and reduced detection sensitivity. Eksigent utilized its expertise in microfluidic chip development to develop a sensitive, non-dispersive UV absorbance flow cell. The detection system brings the dynamic range and linearity of conventional detection systems to the capillary format. Furthermore, it offers fully dispersed array-based detection from 200 nm to 380 nm, providing full spectral information for each peak.
microLC 200 System
The worlds only dedicated splitless microflow UHPLC system designed to deliver optimal micro flow performance and streamlined integration for your most challenging separations - on any mass spectrometry platform.